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Read the percent transmittanceand record it as Time 0 in Table 2.15.

al, 2005) When observing photosynthesis in isolated chloroplasts, DPIP can be used as an indicator – when reduced by the addition of electrons, DPIP turns from a dark blue color to clear, allowing a greater percentage of light to pass through (Hickey, 2000) The purpose of the experiment was to observe the effects of light/dark conditions and boiling on the percentage of light transmission through various chloroplast samples, and thus measure the effects of the same conditions on the rate of photosynthesis.

 In this experiment we were to use a dye reduction technique to study photosynthesis.

Since the percentage of light transmitted increased so dramatically over time, the scientists conclude that the DPIP was reduced (causing the solution to turn clear) by the addition of electrons produced by the light-dependent reactions of photosynthesis.

Read the percent transmittanceand record the data in Table 2.

Theory: Before I predict what will happen I must look at how photosynthesis occurs.

a. Using Excel, construct and label a graph showing the results for the three samples.
b. Identify and explain the control or controls for this experiment.
c. The differences in the curves of the graphed data indicate that there were differences in the number of electrons produced in the three samples during the experiment. Discuss how electrons are generated in photosynthesis and why the three samples gave different transmittance results.

3. A controlled experiment was conducted to analyze the effects of darkness and boiling on the photosynthetic rate of incubated chloroplast suspensions. The dye reduction technique was used. Each chloroplast suspension was mixed with DPIP, an electron acceptor that changes from blue to clear when it is reduced. Each sample was placed individually in a spectrophotometer and the percent transmittance was recorded. The three samples used were prepared as follows:

Read the percent transmittanceand record it as Time 0 in Table 2.19.

Photosynthesis transforms solar energy into chemical bond energy of carbohydrates.

If the chloroplasts are unboiled and exposed to light, then they will have a higher percent transmittance over time because light will be present and temperature regulated.

From these data, the scientists concluded that, after being tested as independent variables in a controlled experiment, lack of light and boiling of the chloroplasts prevented the chloroplasts from carrying out the light-dependent reactions of photosynthesis, to produce high-energy electrons and reduce DPIP.

We took a reading of the percent of light transmittance and recorded the data in the data table.
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Read the percent transmittanceand record the data in Table 2.

The clearest trend in the results was that over the total time period of fifteen minutes, the mixture of unboiled chloroplasts placed in the light consistently allowed the transmittance of the most light – the trendline for the unboiled/chloroplast mixture when time was graphed against the percentage of light transmitted had a slope 89.8% steeper than any of the other trendlines.

Spectrophotometry: Transmittance and Absorbance | …

Big Ideas 1, 2, 4: Studying Photosynthesis and Respiration can be difficult for many students, but being able to see the process with Vernier Probes will help you understand these scientific processes. Evolution might seem far away from this discussion; however, C4 and CAM plants represent structural and biochemical adaptations for photosynthesis in hot and dry climate and Cytochrome C variation can be used to construct models of evolutionary relationships. The theme of energy transformation is obvious as plants transform light energy into chemical energy. We will spend time discussing the structure of the mitochondrion and the chloroplast because their function is closely related. At the metabolic level, photosynthesis and cellular respiration are mutually symbiotic which shows the theme of interdependence in nature.

The percent transmittance grew to steadily higher numbers as ..

If the spinach leaves are not exposed to CO2, then they will not photosynthesize, because photosynthesis needs CO2 to work.
Background Information
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By: Karly Burton, Eva McDonnell
Photosynthesis Lab
The photosynthesis equation is:
CO2 + H2O + Light C6H12O6 + O2
Photosynthesis is the process that a plant uses sunlight to synthesize food from CO2 and H2O.

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