Call us toll-free

Quick academic help

Don't let the stress of school get you down! Have your essay written by a professional writer before the deadline arrives.

Calculate the price


275 Words


siRNAs and PCR primers used in thisstudy.

Gaithersburg, MD /PRNewswire/ -- Sirnaomics, Inc. () announced today that the company has licensed a polypeptide nanoparticle technology invented by Professor A. James Mixson of University of Maryland Medical School. The licensing agreement grants Sirnaomics exclusive rights to a patent covering use of Histidine-Lysine polymer (HKP) for siRNA therapeutics in the areas of wound healing and ocular diseases (U.S. patent application 60/173,576 filed December 29, 1999). Sirnaomics, Inc., a biopharmaceutical company founded in early 2007, is dedicated to advancing RNA interference (RNAi) technology for novel targeted therapeutic development. The company's multi-targeted siRNA therapeutic programs utilizing its nanoparticle-enhanced delivery technologies represent a unique approach for truly realizing the advantages of small interfering RNA (siRNA)-based drugs to treat various critical human diseases.

siRNAs and PCR primers used in thisstudy.
Photo provided by Flickr

The above findings were further validated by examining the EGFP expression in homogenized cell lysates supplied with protease inhibitors using the western-blot method. Among the aforementioned six test samples, the LMWP-PEG-S-S-siRNA conjugate once again yielded the strongest gene silencing effect on MDA-MB-231-EGFP cells (marked as Sample #6 in Fig. D), with the EGFP down-regulating ratio being about 83.5%; consistent with the fluorescent intensity and FACS analysis data. However, the charge-associated LMWP/siRNA aggregates (Sample #5; Fig. D) and cationic lipofecter/siRNA complex (Sample #4; Fig. D) also displayed comparatively low gene down-regulating efficiency, at a level of 51.5% and 23.7%, respectively. For comparison, flow cytometry examination of the non-reducible LMWP-PEG-S-Mal-siRNA (anti-EGFP siRNA) conjugate (Conj-2) was also carried out. As depicted in Fig. E, Conj-2 yielded a much weaker down-regulation on EGFP expression, reducing its level to 68.3% of the baseline total; as compared to that (21.2%) by the cytosol-reducible LMWP-PEG-S-S-siRNA (anti-EGFP siRNA) conjugate (Sample #6 in Fig. B). This finding demonstrated the importance of retaining the cell-delivered siRNA in the cytosolic compartment, should one want to achieve the most potent gene-silencing efficacy.

RNAi: siRNA Oligo Synthesis_Shanghai Wansheng …

23. Presente A, Dowdy SF. PTD/CPP peptide-mediated delivery of siRNAs.  2013;19:2943-7
Photo provided by Flickr

Purification of the desired LMWP-PEG-S-S-siRNA conjugates was achieved via an anion exchange HiTrapTM DEAE FF (1ml, GE Healthcare, Sweden) chromatography using 20 mM sodium phosphate solution containning 10 mM EDTA at pH 7.2 (solution A) as the equilibrium buffer and the same solution containing 1M NaCl (solution B) as the elution buffer. The optimum elution conditions were as follows: Flow rate: 1 ml/min; Linear Gradient: 10-70% of solution B; Detection Wavelength: 260 nm. For comparison, the retention times on the DEAE column of LMWP-PEG-SH and siRNA were examined under identical conditions, with detection wavelength being set at 215nm for LMWP-PEG-SH.

The Lipofecter/siRNA complex was produced by blending 5μl Lipofecter with 2μg siRNA according to the protocol provided by the supplier. The physical mixtures of LMWP-PEG/siRNA and LMWP/siRNA were prepared by adding equal moles of either LMWP-PEG or unmodified LMWP to siRNA (100 nM) prepared in the DEPC buffer containing 0.15 M NaCl and 10 mM EDTA at pH 7.2. The reaction mixtures were incubated for 5 minutes and then centrifuges at 1200 rpm to collect the final products.

HP Custom siRNA - QIAGEN Online Shop

37. Beverly MB. Applications of mass spectrometry to the study of siRNA.  2011;30:979-98
Photo provided by Flickr

The effect of PCSK9 on the inflammatory response ofmacrophages induced by oxLDL remains unknown. The present studyindicates that PCSK9 affects the biological effect induced by oxLDLin THP-1-derived macrophages. Therefore, we used THP-1-derivedmacrophages to examine the effect of oxLDL on PCSK9 expression. Therole of PCSK9 in the oxLDL-induced inflammatory response of thesemacrophages was examined using small interfering RNA (siRNA) toknockdown PCSK9 expression.

As known the PEG polymer chain, due to its dynamic spatial movement, could shield its attached cargo from recognition and interaction by the host immune system, we developed an innovative strategy to formulate an 1:1 monomeric LMWP (note: a well-established CPP [])-siRNA covalent conjugate via a cytosol-cleavable disulfide linkage (Fig, 1A), without confronting the charge-induced neutralization and aggregation by the two oppositely charged substrates. Presented herein were cell culture results that offered a proof-of-concept demonstration of the plausibility of this chemical conjugation method. Comparing with the charge-complexed CPP-siRNA aggregated product, this chemical conjugate displayed far superior efficacy in delivering fully functional siRNA into the cytosolic compartment in executing presumably the most operational gene-silencing event (Fig. B).

53. Eguchi A, Dowdy SF. siRNA delivery using peptide transduction domains.  2009;30:341-5
Photo provided by Flickr
Order now

    As soon as we have completed your work, it will be proofread and given a thorough scan for plagiarism.


    Our clients' personal information is kept confidential, so rest assured that no one will find out about our cooperation.


    We write everything from scratch. You'll be sure to receive a plagiarism-free paper every time you place an order.


    We will complete your paper on time, giving you total peace of mind with every assignment you entrust us with.


    Want something changed in your paper? Request as many revisions as you want until you're completely satisfied with the outcome.

  • 24/7 SUPPORT

    We're always here to help you solve any possible issue. Feel free to give us a call or write a message in chat.

Order now

High-Yield Synthesis of Monomeric LMWP(CPP)-siRNA …

Human breast cancer cell line MDA-MB-231 cells were utilized for these studies. Briefly, cells were grown at 37 °C in humidified atmosphere in L15 medium supplemented with 1% antibiotics, 1% NEAA and 10 % FBS, according to a previously established protocol []. Formulations containing FITC-labeled siRNA (including: PBS, native siRNA, Lipofecter/siRNA complex, LMWP-PEG/siRNA physical mixture, LMWP/siRNA physical mixture, the LMWP-PEG-S-S-siRNA (Conj-1) and LMWP-PEG-mal-S-siRNA (Conj-2) were then incubated the MDA-MB-231 cells with at 37 °C for 2 h. Cells were washed twice with PBS, fixed with 4% paraformaldehyde for 30 min, treated with the DAPI (2μg/ml) nucleic acid staining, and then subjected to confocal laser scanning microscopy analysis using an Olympus FV-300 laser scanning microscope operated with FLUOVIEW software (Olympus, Tokyo, Japan).

Top siRNA companies | VentureRadar

The EGFP stable transfected cell line MDA-MB-231-EGFP cells were used for these studies. Briefly, the cells were grown at 37 °C in humidified atmosphere in L15 medium supplemented with 1% antibiotics, 1% NEAA and 10 % FBS. inhibition on EGFP expression was evaluated by treating the cells with formulations containing PBS, negative control siRNA, naked siRNA, lipofecter/siRNA complex, LMWP/siRNA physical mixture, the bio-reducible Conj-1, or the non-reducible Conj-2). The concentration of siRNA was maintained at 50 nM in all of these formulations. The treated cells were incubated for 12h, followed by the replacement with fresh culture media and further incubation for up to 3 days at 37 °C. The siRNA gene silencing down effect were assessed by the inhibition on EGFP expression, using different methodologies including confocal microscopy, FACS, fluorescence intensity and Western-blot analysis. Experimental details were described in each of the following sections.

PCSK9 siRNA suppresses the inflammatory response induced …

The particle sizes of the physical mixtures of LMWP/siRNA and LMWP-PEG/siRNA, as well as the LMWP-PEG-S-S-siRNA covalent conjugate were measured using Brookhaven Goniometer Light Scattering system, with deionized water as reference. Samples were prepared in light-scattering vials at the same concentration of siRNA. Dynamic light scattering was performed in high resistivity water as indicated below. All data points for dynamic light scattering were the average of three measurements performed on the same sample, and error bars represented the standard deviation.

While siRNA silencing requires an exact match to its target ..

The company has all the internationally leading core technologies of siRNA chemical synthesis, which include the RNA monomer synthesis technology, the custom and chemically-modified siRNA oligo synthesis technology, the nucleic acid fluorescent labeling technology, and many nucleotide chemical modification technologies.

Order now
  • You submit your order instructions

  • We assign an appropriate expert

  • The expert takes care of your task

  • We send it to you upon completion

Order now
  • 37 684

    Delivered orders

  • 763

    Professional writers

  • 311

    Writers online

  • 4.8/5

    Average quality score

Order now
  • Kim

    "I have always been impressed by the quick turnaround and your thoroughness. Easily the most professional essay writing service on the web."

  • Paul

    "Your assistance and the first class service is much appreciated. My essay reads so well and without your help I'm sure I would have been marked down again on grammar and syntax."

  • Ellen

    "Thanks again for your excellent work with my assignments. No doubts you're true experts at what you do and very approachable."

  • Joyce

    "Very professional, cheap and friendly service. Thanks for writing two important essays for me, I wouldn't have written it myself because of the tight deadline."

  • Albert

    "Thanks for your cautious eye, attention to detail and overall superb service. Thanks to you, now I am confident that I can submit my term paper on time."

  • Mary

    "Thank you for the GREAT work you have done. Just wanted to tell that I'm very happy with my essay and will get back with more assignments soon."

Ready to tackle your homework?

Place an order