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Oligo Analysis Tool - Eurofins Genomics
Nucleic probe (French : sonde nucléique) DNA or RNA sequence marked by a fluorescent isotope or enzyme used to detect homologue sequences by in situ or in vitro hybridization.
Collectively, these data suggested the following: 1) the 4.8 kb (band A) transcript encompasses all four ORF's (serp1129, serp1130, dnaG, and sigA) and this transcript is detectable only when the cells are in exponential phase; 2) sigA is encoded within two unique transcripts (bands C and D) that are highly expressed throughout the growth phase (although expression is downregulated at 10-12 hours of growth); 3) serp1129 and serp1130 are encoded on a separate transcript (band B; or, alternatively, transcript A is prematurely terminated) that is expressed only in exponential phase; and 4) based on the size of transcripts E and F (3.0 kb and 2.5 kb, respectively), the size of the entire MMSO operon (4.8 kb), and the fact that all four probes hybridized to bands E and F, we could not determine the most probable location of these transcripts.
Review (1 page) Concept 2: The Lactose Operon
Finger printing (French : cartographie peptidique) Analytical method that supplies an exact identity card of a protein molecule containing electrophoretic and chromatographic properties of various polypeptide segments of the molecule.
Chaperones also facilitate the target protein'sproper folding, translocation and assembly within cells, preventinginappropriate interactions with other proteins.
Oligo Analysis Tool - Welcome to Eurofins Genomics
Repressor (French : répresseur) Protein synthesised by a regulator gene, which, by binding to a specific site on DNA, the operator gene of an operon, prevents the formation of messenger RNA by the operon's other structural genes and hence stops protein -enzyme- synthesis.
Plasmide Ti (French : plasmide Ti) Plasmid carried by the bacteria Agrobacterium rhizo-genes that has a DNA segment transfeerable and has the property to integrated into the DNA of a vegetable host cell . Ti means tumor inductor.
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Only one strand of a given operon’s DNA is used as a ..
Open reading frame (French : cadre de lecture ouvert, ORF) The sequence of DNA or RNA located between the start-code sequence, or initiation codon and the stop-code sequence, or termination codon.
were mixed with a linear DNA fragment from the streptomycin operon ..
DNA synthetizer (French : synthétiseur d'ADN) An automated machine design to synthesize short polynucleotide chains -oligonucleotides- similar in structure to DNA.
Oligo Synthesis - ELIM BIOPHARM
Phage template (French : phage tempéré) Phage genome can be integrated into the host cell DNA and transform its properties. 1- if integrated into the host cell genome it is called prophage and 2- the template phage has the capacity to lyse the bacteria that it infects.
The Lactose Operon—A Look at Regulation
A single change in the genetic code for this protein, in the sector used for defining the herbicide attachment, deprives the herbicide of attachability and therefore of its herbicidal properties.
Molecular Biology | Biochemistry for Medics – Lecture …
Biotechnology (French : biotechnologie) A set of biological techniques developed through basic research and now applied to research and product development. In particular, the use by industry of recombinant DNA, cell fusion, and new bioprocessing techniques.
Transcription (biology) - Wikipedia
The open reading frame of S. epidermidis serp1129 was amplified using primers 731 and 732 that contained an NcoI and BamHI restriction sites, respectively. The resulting 962 bp product was then digested with BamHI and NcoI and ligated into the BamHI and NcoI sites of pET30a+ vector (Novagen). The resulting plasmid (pNF174) was electroporated into E. coli BL21-DE3 (Novagen) for protein production. The plasmid sequence was verified by sequencing in both directions by the University of Nebraska Medical Center (UNMC) Eppley Molecular Biology Core Facility.
DNA & RNA Oligonucleotides - Quality You Can Trust
Replication of the bacterial chromosome is a complex process requiring the interaction of a variety of essential enzymes including primase, helicase, and DNA polymerases I and III . It is hypothesized that bacteria co-regulate the expression of these unlinked genes to ensure the necessary proteins are available during chromosomal replication. To better understand these processes, the transcriptional regulation of the macromolecular synthesis operon (MMSO) , which contains dnaG (primase), was studied in Staphylococcus epidermidis.
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