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Significance Tests / Hypothesis Testing
After you do a statistical test, you are either going to reject or accept the null hypothesis. Rejecting the null hypothesis means that you conclude that the null hypothesis is not true; in our chicken sex example, you would conclude that the true proportion of male chicks, if you gave chocolate to an infinite number of chicken mothers, would be less than 50%.
(Hint: You should reject the null hypothesis when the percentage inthe box in the center of the distribution is less than 2.5%.)The above exercise is very similar to how a researcher uses anindependent samples ttest.
Hypothesis testing is vital to test patient outcomes.
Since the biologist's test statistic, t* = 4.60, is less than 1.6939, the biologist rejects the null hypothesis. That is, the test statistic falls in the "critical region." There is sufficient evidence, at the α = 0.05 level, to conclude that the mean height of all such sunflower seedlings is less than 15.7 cm.
If the biologist set her significance level α at 0.05 and used the critical value approach to conduct her hypothesis test, she would reject the null hypothesis if her test statistic t* were less than 1.6939 (determined using statistical software or a ttable):
That’s How to State the Null Hypothesis!
In the figure above, I used the to calculate the probability of getting each possible number of males, from 0 to 48, under the null hypothesis that 0.5 are male. As you can see, the probability of getting 17 males out of 48 total chickens is about 0.015. That seems like a pretty small probability, doesn't it? However, that's the probability of getting exactly 17 males. What you want to know is the probability of getting 17 or fewer males. If you were going to accept 17 males as evidence that the sex ratio was biased, you would also have accepted 16, or 15, or 14,… males as evidence for a biased sex ratio. You therefore need to add together the probabilities of all these outcomes. The probability of getting 17 or fewer males out of 48, under the null hypothesis, is 0.030. That means that if you had an infinite number of chickens, half males and half females, and you took a bunch of random samples of 48 chickens, 3.0% of the samples would have 17 or fewer males.
The primary goal of a statistical test is to determine whether an observed data set is so different from what you would expect under the null hypothesis that you should reject the null hypothesis. For example, let's say you are studying sex determination in chickens. For breeds of chickens that are bred to lay lots of eggs, female chicks are more valuable than male chicks, so if you could figure out a way to manipulate the sex ratio, you could make a lot of chicken farmers very happy. You've fed chocolate to a bunch of female chickens (in birds, unlike mammals, the female parent determines the sex of the offspring), and you get 25 female chicks and 23 male chicks. Anyone would look at those numbers and see that they could easily result from chance; there would be no reason to reject the null hypothesis of a 1:1 ratio of females to males. If you got 47 females and 1 male, most people would look at those numbers and see that they would be extremely unlikely to happen due to luck, if the null hypothesis were true; you would reject the null hypothesis and conclude that chocolate really changed the sex ratio. However, what if you had 31 females and 17 males? That's definitely more females than males, but is it really so unlikely to occur due to chance that you can reject the null hypothesis? To answer that, you need more than common sense, you need to calculate the probability of getting a deviation that large due to chance.
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How to Set Up a Hypothesis Test: Null versus Alternative
A fairly common criticism of the hypothesistesting approach to statistics is that the null hypothesis will always be false, if you have a big enough sample size. In the chickenfeet example, critics would argue that if you had an infinite sample size, it is impossible that male chickens would have exactly the same average foot size as female chickens. Therefore, since you know before doing the experiment that the null hypothesis is false, there's no point in testing it.
All null hypotheses include an equal sign in them.
In the olden days, when people looked up P values in printed tables, they would report the results of a statistical test as "PPP>0.10", etc. Nowadays, almost all computer statistics programs give the exact P value resulting from a statistical test, such as P=0.029, and that's what you should report in your publications. You will conclude that the results are either significant or they're not significant; they either reject the null hypothesis (if P is below your predetermined significance level) or don't reject the null hypothesis (if P is above your significance level). But other people will want to know if your results are "strongly" significant (P much less than 0.05), which will give them more confidence in your results than if they were "barely" significant (P=0.043, for example). In addition, other researchers will need the exact P value if they want to combine your results with others into a .
Treat each hypothesis as a twotailed test with a .05 Type I error.
This criticism only applies to twotailed tests, where the null hypothesis is "Things are exactly the same" and the alternative is "Things are different." Presumably these critics think it would be okay to do a onetailed test with a null hypothesis like "Foot length of male chickens is the same as, or less than, that of females," because the null hypothesis that male chickens have smaller feet than females could be true. So if you're worried about this issue, you could think of a twotailed test, where the null hypothesis is that things are the same, as shorthand for doing two onetailed tests. A significant rejection of the null hypothesis in a twotailed test would then be the equivalent of rejecting one of the two onetailed null hypotheses.
Null Hypothesis: Definition  Statistics and Probability
Typically in a hypothesis test, the claim being made is about a population (one number that characterizes the entire population). Because parameters tend to be unknown quantities, everyone wants to make claims about what their values may be. For example, the claim that 25% (or 0.25) of all women have varicose veins is a claim about the proportion (that’s the ) of all women (that’s the ) who have varicose veins (that’s the — having or not having varicose veins).
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