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Symbol for alternative hypothesis.

Usually, the null hypothesis is boring and the alternative hypothesis is interesting. For example, let's say you feed chocolate to a bunch of chickens, then look at the sex ratio in their offspring. If you get more females than males, it would be a tremendously exciting discovery: it would be a fundamental discovery about the mechanism of sex determination, female chickens are more valuable than male chickens in egg-laying breeds, and you'd be able to publish your result in Science or Nature. Lots of people have spent a lot of time and money trying to change the sex ratio in chickens, and if you're successful, you'll be rich and famous. But if the chocolate doesn't change the sex ratio, it would be an extremely boring result, and you'd have a hard time getting it published in the Eastern Delaware Journal of Chickenology. It's therefore tempting to look for patterns in your data that support the exciting alternative hypothesis. For example, you might look at 48 offspring of chocolate-fed chickens and see 31 females and only 17 males. This looks promising, but before you get all happy and start buying formal wear for the Nobel Prize ceremony, you need to ask "What's the probability of getting a deviation from the null expectation that large, just by chance, if the boring null hypothesis is really true?" Only when that probability is low can you reject the null hypothesis. The goal of statistical hypothesis testing is to estimate the probability of getting your observed results under the null hypothesis.

The null hypothesis, in this case, is that themean linewidth is 500 micrometers.

If the teststatistic is greater than the upper critical value or less thanthe lower critical value, the null hypothesis is rejected becausethere is evidence that the mean linewidth is not 500 micrometers.

Null hypothesis - Biology-Online Dictionary

The nullhypothesis, in this case, is that the mean lifetime is greater thanor equal to 500 hours.

This number, 0.030, is the P value. It is defined as the probability of getting the observed result, or a more extreme result, if the null hypothesis is true. So "P=0.030" is a shorthand way of saying "The probability of getting 17 or fewer male chickens out of 48 total chickens, IF the null hypothesis is true that 50% of chickens are male, is 0.030."

After you do a statistical test, you are either going to reject or accept the null hypothesis. Rejecting the null hypothesis means that you conclude that the null hypothesis is not true; in our chicken sex example, you would conclude that the true proportion of male chicks, if you gave chocolate to an infinite number of chicken mothers, would be less than 50%.

Alternative hypothesis - Biology-Online Dictionary

We may doubt thatthe null hypothesis is true, which might be why we are "testing" it.

Here are three experiments to illustrate when the different approaches to statistics are appropriate. In the first experiment, you are testing a plant extract on rabbits to see if it will lower their blood pressure. You already know that the plant extract is a diuretic (makes the rabbits pee more) and you already know that diuretics tend to lower blood pressure, so you think there's a good chance it will work. If it does work, you'll do more low-cost animal tests on it before you do expensive, potentially risky human trials. Your prior expectation is that the null hypothesis (that the plant extract has no effect) has a good chance of being false, and the cost of a false positive is fairly low. So you should do frequentist hypothesis testing, with a significance level of 0.05.

A Bayesian would insist that you put in numbers just how likely you think the null hypothesis and various values of the alternative hypothesis are, before you do the experiment, and I'm not sure how that is supposed to work in practice for most experimental biology. But the general concept is a valuable one: as Carl Sagan summarized it, "Extraordinary claims require extraordinary evidence."

The alternative hypothesis might, in fact, be what we believe to betrue.
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Learn About Null Hypothesis and Alternative Hypothesis

Largediscrepancies between reality and the null hypothesis are easierto detect and lead to small errors of the second kind; while smalldiscrepancies are more difficult to detect and lead to large errorsof the second kind.

you reject the null hypothesis and accept an alternative ..

One of the main goals of statistical hypothesis testing is to estimate the P value, which is the probability of obtaining the observed results, or something more extreme, if the null hypothesis were true. If the observed results are unlikely under the null hypothesis, your reject the null hypothesis. Alternatives to this "frequentist" approach to statistics include Bayesian statistics and estimation of effect sizes and confidence intervals.

What is a Null Hypothesis? - Definition & Examples - …

There are different ways of doing statistics. The technique used by the vast majority of biologists, and the technique that most of this handbook describes, is sometimes called "frequentist" or "classical" statistics. It involves testing a null hypothesis by comparing the data you observe in your experiment with the predictions of a null hypothesis. You estimate what the probability would be of obtaining the observed results, or something more extreme, if the null hypothesis were true. If this estimated probability (the P value) is small enough (below the significance value), then you conclude that it is unlikely that the null hypothesis is true; you reject the null hypothesis and accept an alternative hypothesis.

Examples Of Null And Alternative Hypothesis In Biology

The null hypothesis is a statement that you want to test. In general, the null hypothesis is that things are the same as each other, or the same as a theoretical expectation. For example, if you measure the size of the feet of male and female chickens, the null hypothesis could be that the average foot size in male chickens is the same as the average foot size in female chickens. If you count the number of male and female chickens born to a set of hens, the null hypothesis could be that the ratio of males to females is equal to a theoretical expectation of a 1:1 ratio.

Examples Of Null And Alternative Hypothesis Biology

The alternative hypothesis is that things are different from each other, or different from a theoretical expectation. For example, one alternative hypothesis would be that male chickens have a different average foot size than female chickens; another would be that the sex ratio is different from 1:1.

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