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Metronidazole is bactericidal. The drug affects DNA replication by inhibiting nucleic acid synthesis. The activity of metronidazole against Trichomonas, amoebae and Giardia is also likely to be attributable to disruption of existing DNA and inhibition of its synthesis in those organisms.

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Treat for 10 days.To prepare medicated fish food with flake, FD, or frozen fish food;
Use One Measure (2-5 gallon "in tank" dose) of Metronidazole per 15 minute fish food soaking for an average 60 gallon bio load aquarium (I use "just enough" water to mix fish food and Neomycin).
After soak, pour entire contents into aquarium.Metronidazole is often used in treating early stages or for prevention of Neon Tetra & FNT Disease.
Reference:
ABOUT/ USE: An easily absorbed antibiotic, it is used to treat many sensitive gram–negative and some gram–positive bacteria.
Kanamycin is a water-soluble aminoglycoside antibiotic that is derived from the bacterium Streptomyces kanamyceticus and has a very low adsorption rate in the intestine of animals including fish.
Aminoglycosides work by binding to the bacterial 30S ribosomal subunit, causing misreading of t-RNA, leaving the bacterium unable to synthesize proteins vital to its growth.
Aminoglycosides such as Kanamycin are useful in infections involving aerobic gram-negative bacteria such as Columnaris (especially when combined with Nitrofurazone).Kanamycin works especially well in salt water aquariums.

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Metronidazole is one of the rare examples of a drug developed as antiparasitic which has since gained broad use as an antibacterial agent. Briefly, at Rhone-Poulenc labs, extracts of Streptomyces spp. were screened for activity against , a cause of vaginal itching. Azomycin, a nitroimidazole, was identified, and metronidazole, a synthetic derivative, was used to treat chronic trichomonad infections, beginning in 1959.

Metronidazole was synthesized by France's Rhone-Poulenc laboratories and introduced in the mid-1950s under the brand name Flagyl. It was the first member of the group of drugs that are now called nitroimidazoles. In the US it was licensed to Searle.

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