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Schematic of chemiluminescence enzyme immunoassay procedures

35. Botchkareva AE, Eremin SA, Montoya A, Manclus JJ, Mickova B, Rauch P, Fini F, Girotti S. Development of chemiluminescent ELISAs to DDT and its metabolites in food and environmental samples. 2003;283:45-47

The rate law is d[(A)]/dt = k1[luminol][NaOH]2 – k-1[(A)][H2O]2 = 0

Forensic scientists use the reaction of luminol to detect blood at crime scenes. A mixture of luminol in a dilute solution of hydrogen peroxide is sprayed onto the area where the forensic scientists suspect that there is blood. The iron present in the haem unit of haemoglobin (see ) in the blood acts as a in the reaction described in . The room must be dark and if blood is present, a blue glow, lasting for about 30 seconds, will be observed. The forensic investigators can record this glow by using photographic film, which can be used as evidence in court for the presence of blood at the scene. (For a teaching activity about forensic science, see .

For a further investigation into luminol, try investigating

Calibration graph of microplate chemiluminescence enzyme immunoassay for FT4

One of the drawbacks of using luminol is that the reaction can be catalysed by other chemicals that may be present at the crime scene, for example, copper-containing alloys, some cleaning fluids such as bleach, and even horseradish. Clever criminals can clean up the blood with bleach, which destroys the evidence of the blood, but bleaching the carpet may alert people to the crime sooner. Urine also contains small amounts of blood, which can be enough to catalyse the reaction of luminol. Once luminol has been applied to the area, it may prevent other tests from being performed there. However, despite these drawbacks, luminol is still used by forensic scientists as a tool to solve crime.

c. Have the students look over the materials, procedure, and lab safety precautions on the Kinetics of the Chemiluminescent Reaction of Luminol lab handout. Have the students develop their own procedures and record them in their graphic organizers. Remind the students that they will have only the next class period to conduct their experiments and to plan accordingly. Before having the students start their experimentation, have them turn in their procedures so that any major errors can be corrected. [40 minutes]

The Chemiluminescence of Luminol - Home

The pH chemistry of luminol chemiluminescence is very complicated and I failed to get any kind of conclusive data from my own experiments.

There have been a number of experiments investigating aequorin, a protein found in certain jellyfish, which produces blue light in the presence of calcium (see , and ) and can thus be used in molecular biology to measure calcium levels in cells. Some scientists have come up with other ideas for utilising bioluminescence in the future, for example self-illuminated Christmas trees. Can you think of any other exciting potential uses for this amazing natural phenomenon?

Bioluminescent reactions use (adenosine triphosphate) as a source of energy. The structure of the light-producing molecules varies from species to species, but they are all given the generic name luciferin. The structure of firefly luciferin is shown in , left. When fireflies glow, the luciferin is oxidised to produce an excited complex, which falls back down to the ground state, releasing a photon of light, just like the chemiluminescent reaction of luminol described in . However, fireflies do not use hydrogen peroxide and potassium hexacyanoferrate(III) to oxidise luciferin; instead they use molecular oxygen and an enzyme called luciferase (this is also a generic name – luciferases vary from species to species).

I found chemiluminescence to only appear at pHs higher than 8-9 and found luminol to only dissolve easily at approximately 11.
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Luminol Lab Report - Term Paper

Luminol, also known as 3-Aminophthalic hydrazide, is a chemical that exhibits chemiluminescence that reacts with iron found in hemoglobin. Biologists use luminol in cellular assays for the detection of iron, copper, and cyanides. Ungraded products supplied by Spectrum are indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or therapeutic use. These materials may or may not have a Certificate of Analysis available.

01/12/2014 · Read this essay on Luminol Lab Report

Luminol, also known as 3-Aminophthalic hydrazide, is a chemical that exhibits chemiluminescence that reacts with iron found in hemoglobin. Biologists use luminol in cellular assays for the detection of iron, copper, and cyanides. Ungraded products supplied by Spectrum are indicative of a grade suitable for general industrial use or research purposes and typically are not suitable for human consumption or therapeutic use. These materials may or may not have a Certificate of Analysis available.

Synthesis and Chemiluminescence of Luminol - …

The article can be adapted for different age ranges and for different subjects and topics. For students aged 14-15, it could be used to teach chemistry (atomic structure and movement of electrons between shells, introduction to chemical reactions) or biology (bioluminescence). For this age group, the teacher would need to simplify the information in the article and omit the details of the reactions. For students aged 16-18, the article could be used to teach chemistry (redox reactions, catalysts, the influence of temperature on reaction speed, the effect of pH on a reaction, and covalent bonds), physics (the electromagnetic spectrum and photons) or genetics (genetic engineering). Suitable comprehension questions include:

Chemiluminescence - the oxidation of luminol | …

Luminescence: The production of light, usually at low temperatures, for example by chemical reactions or electrical energy. Incandescence, in contrast, is light generated by high temperatures.

Crime Scene Chemistry—The Cool Blue Light of Luminol

The specificity of the immunoassay was dependent on the antibody's specificity []. The cross-reactivity of the anti-T4 antibody was evaluated using several T4 disrupting compounds. 3,5,3'-triiodothyronine (T3) and 3,3',5'-triiodothyronine (reverse T3, rT3) were the structurally related compounds of T4 (Figure ) and were tested in this work. The cross-reactivity was calculated by deriving a ratio between does of interfering substance to does of thyroxine needed to displace the same amount of the conjugate. The results were shown in Table . The antibody exhibited cross-reactivity all less than 0.01%. Therefore, effects of T3 and rT3 in serum on FT4 determination could be neglected.

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