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Tib Molbiol - Oligonucleotides, Design for PCR and …
coli Bidirectional replication of molecules give rise to and finally two General Scheme for In vitro Enzymatic Replication of DNA
Chemistry of reaction catalyzed by DNA Polymerase.
If a person were to attempt extending a synthetic oligonucleotide prepared to be complementary to a target on human DNA by just one base, using DNA polymerase and dideoxynucleoside triphosphates, using four different tubes each containing all four bases, but only one of them in each tube alpha-labeled with 32P, optimistically one might be able to discover the identity of the nucleotide on the DNA target just three-prime of the oligomer. Dideoxy-sequencing worked that way…but…Huge but…that only worked on cloned DNA where the ratio of target to non-target DNA was increased by a factor of about a million. Fortunately for me I was thinking about other things that might go wrong than just the brute improbability that only the right sequence would be engaged. I paid just enough attention to this hypothetical problem to plan on using two oligonucleotides, one designed for each strand of the target sequence coming at the base pair in question from either side. Although these two sides would be far distant in the denatured reaction mixture they would still represent complementary strands and if one told me that a 'T' was three-prime to one oligo, the other should have told me 'A' was three-prime to the other. Not much of a control, but I had oligos to burn. In fact that was what I was trying to do. We had excess oligos on our hands.
Polymerase chain reaction - Wikipedia
Dr. Mullis joined the Cetus Corporation in Emeryville, California, as a DNA chemist in 1979. During his seven years there, he conducted research on oligonucleotide synthesis and invented the polymerase chain reaction.
Biography - Dr. Kary Banks Mullis
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