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Aim: To show that chlorophyll is necessary for photosynthesis

Here is a summary of lighting requirements for different aquarium types. I recommend timers for any aquarium to provide good daylight/night cycles, however this is even more important with Planted Freshwater and Saltwater Reef or Nano Reef tanks. Turn the actinic lights on about one to 1/2 hour ahead of the daylight bulbs and one to 1/2 hour later in the evening.
I generally have the brightest lights on for about 12 hours per day, with 1 or maybe 2 hours of less bright or "ramping" up or down of LEDs if used. Sometimes with MH I will have them in a third cycle that is on for only abut 10 hours or less.

Despite commentary in some aquarium keeping forums, there is NO evidence that ramping up and down much longer than 1 hour where strong lighting is used provides ANY benefit to plant growth, fish, or reef environments (this is not applicable where one low to moderate lighting is used and one on/off cycle is all that is needed).
I have personally kept many aquariums (100s) going back to where only timers were all we had and used single time one for strong lighting, partial tank on with full lighting an hour later, and multiple timers. While have partial lights on for an hour did produce results over a strong on/off, multiple cycles made NO difference!
Think of it this way; in tropical regions, there is little difference in bending of light rays from the sun much past an hour after sunrise or an hour before sunset, so thinking a ramp up/down cycle much more than this time will make a difference has no practical or scientific evidence to back this up.

For LED moonlight settings, generally just a 1-5% of your full lighting power setting is sufficient between main lighting cycles.
If you have separate moonlights, I would run these 8-16 hours (I have yet to find in benefit from this that can be scientifically proven other than aesthetics).

ANY fluorescent light used for aquarium applications such as planted aquariums or reef, slowly burns up phosphors and other rare earth elements that produce the light energy necessary for PUR.
As with a UVC bulb/lamp used for a UV Sterilizer, these lights go through a "half life", meaning that a light that is run 12 hours per day that may last 2 years (as per rated life) should be actually replaced every year otherwise these lamps are running at 50% and less of initial light energy production and then often producing much more yellow light and even an imbalance of red that is inducing to more algae and cyanobacteria growth in particular.




The picture to above/left clearly demonstrates the difference we can see with just our human eye between new 6400K Daylight SHO and one nearly two years old.
The color temperature of the old lamp/light.

To prove that chlorophyll is required for photosynthesis; ..

Another term is . I should point out that while the terms PAS & PUR have a lot in common, there is a difference in that PAS is most simply stated as the spectrum where "chlorophyll is much more efficient at using the red & blue spectrums of light to carry out photosynthesis. Therefore, the action spectrum graph would show spikes above the wavelengths representing the colors red & blue."
Reference: .

To prove that carbon dioxide is necessary for photosynthesis ..

26/11/2017 · How do you prove that chlorophyll is essential for photosynthesis

Chlorophyll is the green photosynthetic compound in plants that captures energy from sunlight necessary for photosynthesis. The amount of chlorophyll present in plants plays an important role in determining how healthy they are. Accurately monitoring chlorophyll from space, therefore, provides a valuable tool for modelling terrestrial productivity and support for the managements of vegetated environments.

These same older generation emitters, controller technology & drivers are the reason I did not recommend LEDs of ANY brand for "higher-end" aquarium applications until 2008 (readers of this VERY constantly evolving article in 2007 would note this too).
However NOW we have many reef/planted aquarium capable LED lights and the only thing that separates most are bells & whistles, cost, efficiency of energy input to output, colors one might desire (albeit not necessarily what the photosynthetic life is more efficient utilizing), water proofing/resistance, and warranty. Even in cost, often the other factors will override an initially low cost of many popular LED fixtures.

An experiment to show chlorophyll is necessary for ..

That is why they are used in experiments to prove that chlorophyll is necessary for photosynthesis

But science is always subject to becoming dogmatic and hypotheses can prevail for reasons of wealth, power, rhetorical skill, and the like, not because they are valid. The history of science is plagued with that phenomenon, and probably will be as long as humanity lives in the era of scarcity.As will become a familiar theme in this essay, the rise and fall of species and ecosystems is always primarily an energy issue. The Ediacaran extinction is a good example: Ediacaran fauna either an energy source for early Cambrian predators, ran out of food energy, ran out of the oxygen necessary to power their metabolisms, or lacked some other energy-delivered nutrient. After the extinction events, biomes were often cleared for new species to dominate, which were often descended from species that were marginal ecosystem members before the extinction event. They then enjoyed a of relative energy abundance as their competitors were removed via the extinction event. For this essay’s purposes, the most important ecological understanding is that the Sun provides all of earthly life’s energy, either (all except nuclear-powered electric lights driving photosynthesis in greenhouses, as that energy came from dead stars). Today’s hydrocarbon energy that powers our industrial world comes from captured sunlight. Exciting electrons with photon energy, then stripping off electrons and protons and using their electric potential to power biochemical reactions, is what makes Earth’s ecosystems possible. Too little energy, and reactions will not happen (such as ice ages, enzyme poisoning, the darkness of night, food shortages, and lack of key nutrients that support biological reactions), and too much (such as , ionizing radiation, temperatures too high for enzyme activity), and life is damaged or destroyed. The journey of life on Earth has primarily been about adapting to varying energy conditions and finding levels where life can survive. For the many hypotheses about those ancient events and what really happened, the answers are always primarily in energy terms, such as how it was obtained, how it was preserved, and how it was used. For life scientists, that is always the framework, and they devote themselves to discovering how the energy game was played.

The reader should note from all the information written above, that when deciding what lighting to get for your aquarium that the watts used is only one third or less of the equation in deciding what lights, what size and how many should be used. I will admit that I still will use the watts per gallon as a starting point with SPECIFIC lights comparing apples to apples; however specimen placement or tank depth, lighting type strengths and weaknesses must be considered too.
Do no compare apples to oranges; such as SHO to T12, or High Output LED to low output LED such as Marineland LEDs. Or even comparisons of a super premium high PUR per wattage input LED to other high end LEDs but that still require a higher input wattage for the same results due to energy loss as heat, less than optimum PUR, use of current reduction technology instead of optimum PWM technology (example the AquaRay AquaBeam to the still excellent but higher wattage requiring Maxspect and ZetLight LEDs)



Please note that besides years of personal fresh and saltwater keeping experience, MUCH more of this information I have written here comes from research OUTSIDE the aquarium industry. Much of what I have learned (and I am STILL learning) comes from this constant research of as many lighting tech research as I can read often from horticulture or other outside sources as noted earlier.

Some examples include the lack of information in the aquarium industry/hobby that must be found elsewhere includes the SHO or T2 lights that are often superior to more commonly recommended bulbs in the aquarium hobby,

There is also good evidence that correct lighting benefits ALL fish as well, including salt & freshwater fish. I have observed better disease resistance in marine fish in loosely controlled studies when lighting is upgraded to higher intensity, high PAR/PUR lights. Proper lighting may play a role in nutrient assimilation, improved Redox, lower incidence of Brown Diatom Algae. Studies in humans that show an impact of lighting on health, may have strong implications for fish (this may be a factor in my studies that showed higher disease resistance when lighting is improved).
Reference:

Lighting that as closely duplicates the sun (not necessarily light that is most pleasing to us) is important for ALL life, although more noticeably for corals and plants. Fish too are part of this chain of life. Basically if you take away the sun and the energy it provides, you take away life itself and I do not think if you are trying to achieve the best environment for your fish whether fresh or saltwater, you are doing them a favor by depriving of this source of energy, so duplicating this is one more part of your "aquarium keeping puzzle".
Reference; .

I should point out that obviously, some fish prefer subdued light, but this is easily handled by hiding places, caves, plants (live or artificial), products such as Peat, Pillow Moss, or Indian Almond Leaves that "color" the water, and simple placement of lights where as some areas of the aquarium are better lit than others with plants/corals placed in way that benefit the most in these areas.

As I noted earlier, I am STILL learning after being in the hobby since the late 60s and professionally employed in the aquarium industry since 1978.
who will shoot down the science that is explained here because it does not fit their view and the fact that they are unwilling to learn.
What is amazing to me, is that often many simply confuse what they like with the known facts of science.
Example; just because you might like green and yellow LED emitters, does NOT mean that these wave lengths are as efficient as source of energy for an acropora coral or aquarium plant.
Another act of scientific malpractice I have often noted is the simple fact that energy lost as heat is energy that is not going to light energy, which all lights are guilty of, but to widely varying degrees, yet many choose to ignore this most basic principle of science when comparing lights.
FINALLY, another example would be moon or lunar lights. I myself prefer blue lights for this, as have most of my clients, but this still does not make moon light blue, as the facts are it is NOT!!







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In the experiment to prove chlorophyll is necessary for photosynthesis- Q1 Why is a variegated leaf used Q2 Why is the variegated leaves kept in a dar
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