Call us toll-free

Quick academic help

Don't let the stress of school get you down! Have your essay written by a professional writer before the deadline arrives.

Calculate the price

Pages:

275 Words

$19,50

Genetic Regulations of the Biosynthesis of Microbial Surfactants: ..

Polymorphum gilvum SL003B-26A1T is the type strain of a novel species in the recently published novel genus Polymorphum isolated from saline soil contaminated with crude oil. It is capable of using crude oil as the sole carbon and energy source and can adapt to saline soil at a temperature of 45°C. The Polymorphum gilvum genome provides a genetic basis for understanding how the strain could degrade crude oil and adapt to a saline environment. Genome analysis revealed the versatility of the strain for emulsifying crude oil, metabolizing aromatic compounds (a characteristic specific to the Polymorphum gilvum genome in comparison with other known genomes of oil-degrading bacteria), as well as possibly metabolizing n-alkanes through the LadA pathway. In addition, COG analysis revealed Polymorphum gilvum SL003B-26A1T has significantly higher abundances of the proteins responsible for cell motility, lipid transport and metabolism, and secondary metabolite biosynthesis, transport and catabolism than the average levels found in all other genomes sequenced thus far, but lower abundances of the proteins responsible for carbohydrate transport and metabolism, defense mechanisms, and translation than the average levels. These traits support the adaptability of Polymorphum gilvum to a crude oil-contaminated saline environment. The Polymorphum gilvum genome could serve as a platform for further study of oil-degrading microorganisms for bioremediation and microbial-enhanced oil recovery in harsh saline environments.

 Genetic Regulations of the Biosynthesis of Microbial Surfactants  PDF View and Downloadable.

Firstly, biosurfactants, with glycolipids and lipopeptides reported as the most common ones produced by hydrocarbon degrading microbes , can emulsify and solublize the hydrocarbons to increase the microbial connection with oil components. Genes encoding the key enzymes in glycolipid synthesis in the genome of SL003B-26A1T, include acyltransferase, 3-oxoacyl-(acyl-carrier-protein) reductase, glycosyltransferase, phosphomannomutase (AlgC), and ketoreductase (RhlG) . Three genes encoding OmpA-like proteins found are related to lipopeptide synthesis. The proteins are characteristic with its highly hydrophobic amino acid composition within four putative extra-membrane loops, which were suggested to be the active component of the bioemulsifier alasan , ().

Genetic Regulations of the Biosynthesis of Microbial ..

All rights of this Genetic Regulations of the Biosynthesis of Microbial Surfactants file is reserved to who prepared it.

At the regulation level, 12 LuxR regulator coding genes were identified, including two LasR-LasI TCS systems (SL003B_0642-0643 and SL003B_0701-0702). The LasR regulator was reported to be related to regulation of glycolipid biosynthesis under the autoinducer LasI .

Thirdly, strain SL003B-26A1T has 12 genes for type VI pili assembly, which mediates biofilm formation and microbial adhesion to biotic and abiotic surfaces , , and the oil-water interface. The TCS for flagella biosynthesis and cell motility was also identified, including the response regulators CheY ( and ) activated by the regulators CheA (SL003B_0948), CheW (SL003B_0949), CheB (SL003B_0951), and CheR (SL003B_0952) . Flagella assembly plays an important role in cell motility and chemotaxis, which could also help bacteria move to relatively better niches and attach to the oil-water interface where the degradation of alkanes can take place and more carbon sources are available for growth ( and ).

Microbial Surfactants - [PDF Document]

20/12/2017 · Genetic Regulations of the Biosynthesis of Microbial Surfactants: ..

This module is only available to those students who enter under the B990 programme. The Molecular Clinical Microbiology module will deliver microbiology from a molecular perspective and describe its use in clinical microbiology. The module will develop the theme of basic microbiology and clinical microbiology taught in the level 4 module (the basic microbiology module) and the level 5 module (the clinical microbiology module). It will include the background and techniques to gene expression and regulation, genetic transfer, the synthesis of macromolecules, sub-cellular organization, cell to cell communication, molecular aspects of pathogenicity and virulence and the relationship of these to clinical microbiology. Microbial diversity will be taught at the species level by comparing of 16S RNA sequences and at the subspecies level by MLST. VNTR and SNPS will be taught to index diversity of genetically monomorphic bacteria. Real-time PCR will be taught for laboratory identification of microbial diversity. Bioinformatics will be used to catalogue diversity and genomics used to interpret diversity.

Prerequisites: Basic Biochemistry (SBS017). This module covers a range of topics including: Chemical reactions - Biochemical logic. Biochemistry of some vitamin and coenzyme catalysed reactions. Glycogen synthesis and degradation. Pentose phosphate pathway. Gluconeogenesis. Amino acid metabolism and the urea cycle. Fatty acid synthesis and breakdown. Prostaglandin and steroid biosynthesis. Purine, pyrimidine and deoxyribonucleotide synthesis. Control and regulation of metabolism.

Genetic Regulations of the Biosynthesis of Microbial Surfactants - PDF Viewer You currently don't have Adobe Reader installed
Order now
  • UNMATCHED QUALITY

    As soon as we have completed your work, it will be proofread and given a thorough scan for plagiarism.

  • STRICT PRIVACY

    Our clients' personal information is kept confidential, so rest assured that no one will find out about our cooperation.

  • COMPLETE ORIGINALITY

    We write everything from scratch. You'll be sure to receive a plagiarism-free paper every time you place an order.

  • ON-TIME DELIVERY

    We will complete your paper on time, giving you total peace of mind with every assignment you entrust us with.

  • FREE CORRECTIONS

    Want something changed in your paper? Request as many revisions as you want until you're completely satisfied with the outcome.

  • 24/7 SUPPORT

    We're always here to help you solve any possible issue. Feel free to give us a call or write a message in chat.

Order now

Chapter 33 - Toxicology INTRODUCTION

Analyses of the abundance of the COG categories of Alcanivorax borkumensis SK2, Geobacillus thermodenitrificans NG80-2, and Desulfatibacillum alkenivorans AK-01 revealed similarities and differences among these hydrocarbon-degrading strains. The toxicity and low availability of oil components as carbon sources could be the driving forces for these bacteria to evolve sensitive sensing and response systems to avoid damage by hydrocarbons and pursue nutrients. In the genomes of SL003B-26A1T, SK2, NG80-2, and AK-01, the abundances of protein categories responsible for carbohydrate transport and metabolism are lower than the average value for all genomes in the IMG database. This is in accord with the low carbohydrate availability in the environments where these strains were isolated. Fatty acids are important intermediate products in the alkane degradation pathway, and lipid transport and metabolism are therefore important for the further degradation of alkanes. It is therefore reasonable that the abundances of protein categories responsible for lipid transport and metabolism, and secondary metabolite biosynthesis, transport, and catabolism, are higher in the genomes of SL003B-26A1T, SK2, NG80-2, and AK-01 than the average level in all other genomes. The high abundance of lipid metabolism-related proteins in these strains reveals the genetic basis of the conversion of alkanes to energy. The biosurfactants synthesized as secondary metabolites are also essential in crude oil degradation, in which emulsification of the crude oil could help the strains to utilize hydrocarbons more easily , . Furthermore, it is interesting that although the strain SK2, isolated from marine sediment, could utilize crude oil, proteins in SK2 belonging to cell motility COG categories are fewer than those in SL003B-26A1T and NG80-2, which were isolated from terrestrial oil-contaminated environments. The reason why cell motility is more important for SL003B-26A1T and NG80-2 may be that soil and oil reservoirs are much more compacted.

Order now
  • You submit your order instructions

  • We assign an appropriate expert

  • The expert takes care of your task

  • We send it to you upon completion

Order now
  • 37 684

    Delivered orders

  • 763

    Professional writers

  • 311

    Writers online

  • 4.8/5

    Average quality score

Order now
  • Kim

    "I have always been impressed by the quick turnaround and your thoroughness. Easily the most professional essay writing service on the web."

  • Paul

    "Your assistance and the first class service is much appreciated. My essay reads so well and without your help I'm sure I would have been marked down again on grammar and syntax."

  • Ellen

    "Thanks again for your excellent work with my assignments. No doubts you're true experts at what you do and very approachable."

  • Joyce

    "Very professional, cheap and friendly service. Thanks for writing two important essays for me, I wouldn't have written it myself because of the tight deadline."

  • Albert

    "Thanks for your cautious eye, attention to detail and overall superb service. Thanks to you, now I am confident that I can submit my term paper on time."

  • Mary

    "Thank you for the GREAT work you have done. Just wanted to tell that I'm very happy with my essay and will get back with more assignments soon."

Ready to tackle your homework?

Place an order