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Light Intensity and Rate of Photosynthesis (Carol …
After a shower, the soil is soft and the animals which walked there, leave tracks. Bymeans of plaster, you can obtain the mould of these tracks and make an
interesting collection (figure 9). The experiment is very simple: it is a question ofbringing a little plaster in dry form, some water, a bowl to mix the plaster,
and a spoon to stir. But, to which animal belong the tracks you have picked up? From herestart the research of information. They exist books which give the picture of the tracksof many animals. Moreover, recognizing the animal which left the track is not sufficient:it is necessary to know something of its behavior, etc. From the simple collection oftracks you will be curious to know the animals of your land. Our article: "" provides you some indication on this topic. This experiment will openyour eyes so you can observe the territory around you when you walk in naturalenvironments with more attention. Plaster can be used also to obtain the mould of fruits,barks and other natural objects. From the cast you can also obtain the original shape.
Considering that not all light energy is used for photosynthesis we propose an alternative hypothesis. In a previous experiment the pigment xanthophylls absorbed significant amounts of blue light. In new research it is found that this pigment could be an important component in a process called energy dissipation rather than photosynthesis. In order to not overwhelm the plant with photosynthesis and respiration, this photon energy goes to other functions or formations of the plant. Further research on the function of xanthophylls will need to be conducted in order to understand the processes of plant function.
To show experimentally that light is necessary for photosynthesis.
Drosophila, best known as vinegar fly or fruit fly, is the little fly you see flyingaround vinegar and on fruit during the fall. Why raise vinegar flies? To observe theirdevelopment, to observe the chromosomes of their salivary glands during division, toperform experiments on genetics, finally as food for amphibians that have just completedtheir metamorphosis. In this case it is necessary to breed a species that can't fly. Youcan obtain individuals with vestigial wings (wings which are not fully developed)at a university Biology or natural sciences department.
Culture medium recipe for drosophila: water 83 ml, agar-agar 0.8 g, sugar 5 g, brewer'syeast 10 g, alcohol 1.3 ml, nipagin
0.25 g. Nipagin M is used as a preservative in foods and cosmetics, like agar-agar, youcan buy it at the stores that sell science items for laboratories. Mix the yeast and thesugar, add agar-agar and water and simmer for 3 minutes. Turn off the heat. Dissolve thenipagin in alcohol and add to the rest when it has stopped smoking. Mix and let it set.
You can find other recipes at the following websites:
Aquick and simple introduction to Drosophila melanogaster
Fruit Flies - Drosophila melanogaster
Drosophila Culture (how to culture flightless fruit flies)
Observing the Development of Drosophila in Apple Juice Agar
Drosophila Genetics Lab I
A bibliography for an insect field biology course
La drosophile (in French)
Internet keywords: drosophila culture -cells, wingless fruit flies vinegar fly.
In its simpler form, this experiment is particularly suited to elementary schools.
1 - Put some beans in a little jar containing some moist cotton wool. Keep the jar closedto maintain the humidity. Every day pull out the cotton and observe the state of the seedsand measure the length of their roots.
2 - Students in junior high schools or high schools can try to evaluate the influence ofparameters such as temperature, light and nutrients on the speed of germination of theseeds. If seeds are placed in gelatin, it is possible to observe the germination withoutextracting them from the jar.
3 - You can also collect seeds of different plants and determine their vitality (percentof seeds which germinate) as a function of time to harvest.
Exploring Seed Germination
Germinating Seeds on Gelatin
The Great Seed Mystery For Kids
The Wonderful World of Seeds
Internet keywords: germination seeds student.
Light Intensity and Photosynthesis
If placed in a suitable nutrient environment, cells and tissues of many organisms are ableto reproduce and form new plants or animals. Now, we will deal with vegetable tissues,whose culture is simpler than that of animal cellules and tissues. It is necessary toprepare a nutritive and sterilized culture medium for the piece of plant tissue. Keep theculture in the suitable conditions of light and temperature and which vary from plant toplant. Over many days, you will observe the growth of a callus or roots or shoots. In thisway you can obtain even whole plants (cloning). These experiments show that special cellskeep all the information necessary to generate the whole plant.
As we have mentioned, it is necessary avoid bacteria and moulds in the cultures. For thisyou will need sterilize tools, vials, tubes, and nutrient medium. Place each in anautoclave for a ten minutes or, lacking an autoclave, a pressure cooker. The tissues aswell have to be free from microorganisms and they have to be sterilized with bleach (40%solution for 15 min) or with alcohol.
The transfer of the tissues into the test tubes has to be made in aseptic conditions,using a sterile box. Lacking that, make your first trials in a quiet place, as devoid ofwind and dust as possible. The culture medium should contain water, vitamins (particularlythose of the B-complex. For this, use yeast extract), sugars, mineral salts. To enrich thewater with mineral salts, boil some water with a handful of soil, then let settle andfilter it. Usually, people also insert 0.5-0.8% of agar-agar to "solidify" themedium. As culture medium, coconut milk has been used. It contains mineral salts, sugars,vitamins and growth hormones.
1 - For yours first tests of micropropagation, use strawberries tissues.
2 - If this simple experiment interests you, you can continue on the way of the invitro culture of vegetable tissues. In fact you can propagate a lot of plants in thisway. Plants easy to culture are the following: tomato, potato, strawberry, chrysanthemum,geranium, sunflower, tobacco, carrot and onion. You can use tissues obtained from seeds,such as the embryo, but you can use also tissues taken from adult plants, such as tissuesof roots, stems, apical buds, shoots, leaves, even single cells. Each plant and tissue hasits own needs. They are different from each other. You can try the influence of thevegetable hormones, special nutrients, etc.
This field is very broad and complex so, if you are interested in continuing with theseexperiments, you can buy special books and you should build a sterile box.
Plant Tissue Culture for the Gardener
Basic Principle in Plant Tissue Culture Technique
Plant Tissue Culture Kit Manual
Plant Micropropagation Using African Violet Leaves
Plant Tissue Culture (links)
Internet keywords: in vitro culture plant tissue micropropagation.
The bottle garden is a close relative of the biosphere: a tiny garden growing inside abottle, a glass sphere, or a demijohn. The main difference is that soil is the maincomponent not water. The container can be kept sealed or opened like a terrarium. Ourswill be more for just plants whereas the terrarium may have animals. These gardens in abottle, can be cured and perfected as a system, they are also very decorative, in fact youcan treat them as a bonsai with not only a single tree, but whole landscapes. A carefullychosen stone becomes a rock, a small plot of moss represents a great prairie. A contorted,little plant is now a hundred year old tree. Maybe a marsh wetlands with peat moss,mushrooms and ferns is more to your liking. Maybe appropriate succulent plants with sandand rocks for a vast desert landscape. The choice is yours. There is no rigid rule aboutbeing either an open or a closed garden. It maybe useful to keep the lid on to retainwater. Or eventually, the plants grow up and need to hang down the outside.
If the container is always sealed, the challenge becomes quite demanding. In fact, it thenis necessary to obtain an equilibrium between plants and animals in order to keep aneffective cycle of substances and energy, but if this is difficult in a predominantlywater environment, it is even more difficult in a just moist environment. So, the gardenhas to contain a moist soil, plants, little animals like terrestrial isopods (i.e.: sowbug, pill bug), earthworms, etc. Plants have to be resistant to high moisture and have asmall size and limited growth. To avoid roots rotten, you can also try to refer tohydroponics and substitute the ground with small sized expanded clay or with gravel. Inthis case you will have more difficulties in hosting animal species, so it will have to beleft open. It is important you place your garden in a cool and well illuminated place, butaway from direct sunlight. Open or closed, a bottle garden is a fascinating thing, and theidea itself of a little and self-sufficient world is fascinating as well. There are peopleso fond of bottle gardens they work at them a long time, taking care of them using longand thin tools and to observe these environments till they imagine to be one of theirinhabitants.
Then choose among these main options:
1 - Opened bottle garden, containing moist ground, plants and possible little animals. Itis suited for people who love taking care of gardens and who have a flair for
the architecture of little wide spaces.
2 - Opened bottle garden, prepared in hydroponics and containing plants. It is suited forthose who tend to forget to water their plants.
3 - Sealed bottle garden, holding moist soil, plants, and some little animals. Thisexercise is suitable for people who are keen on biology and scientific research. They willhave to find appropriate plants and animals suitable for a sealed ecosystem and will haveto achieve the necessary conditions for a long survival. This research, bibliographic inpart and experimental, will keep students happily busy for long time.
The educational value of these little ecosystems is evident. They can be carried out evenin a school, where the teacher will be allowed to illustrate their characteristics topupils and encourage interesting discussions. In the web sites below, you will findimportant practical information in building your bottle gardens.
Terrariums : Miniature Worlds in a Bottle!
BBC online, how to gardening, bottle garden
Un jardin en bouteille
Le terrarium : Un monde miniature dans une bouteille!
Internet keywords: garden bottle jar, jardin bouteille bonbonne.
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Draw photosynthesis (light dependent reactions) MONDAY 1/17: ..
Introduction Real World connections Light intensity is a factor in the rate of photosynthesis, which is the production of simple sugars from carbon dioxide and water, causing the release of sugar and oxygen.
Intensity of Light and Rates of Photosynthesis
There are four things that are required by the plant before it can carry out photosynthesis, they are light, chlorophyll, carbon dioxide and water ....
How Photosynthesis Works - Science for Kids
Hypothesis: I hypothesize photosynthesis will be faster under normal light conditions.
To test our theory we set up four Experimental Groups;
Trial 1 : Spinach Discs in cylinder number one will receive no sunlight.
Trial 2: Spinach Discs in cylinder number two will receive strobe light.
Trial 3: Spinach Discs in cylinder number three will receive direct light from a lamp.
Trial 4 ( Control Group): Spinach Discs in cylinder number four will receive natural light.
Photosynthesis Experiments for Kids | Education - …
The Effects of pH Levels and Phosphorous on the Photosynthesis Process of Aquatic PlantsStudy the coniferous spruce tree (Picea glauca as the species of choice) by measuring its lateral and sub-lateral branch angles in order to see how it collects sunlight from various angles on its south-facing side.
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