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Glossary | Linus Pauling Institute | Oregon State University
Annotating the phenotypic outcomes of individual missense mutations is an important but challenging task for understanding the molecular mechanism of multigenic diseases like cancers. Information at both the protein and pathway levels can be used to increase the accuracy of interpreting the phenotypic effects of a target mutation; nevertheless few methodologies are available for this purpose. To tacle this issue, I have explored and validated an multi-level approach, PEPP (Phenotype Extrapolation via Pathway and Protein information), to effectively integrate the structural factors of proteins into pathway dynamics.
The probe signal reflect formation of targetproduct, while melting curve analysis of the BOXTO signal revealsprimer-dimer formation and the presence of any other anomalous products.
Journal of Nanoscience and Nanotechnology
A novel closed-tube method for distinguishing thermophilicNaegleria species is presented, using a single primer set and the DNAintercalating dye SYTO9 for real-time PCR and melting-curve analysis ofthe 5.8S ribosomal DNA gene and flanking noncoding spacers (ITS1,ITS2).
Robert P. Heaney, M.D. | Professor of Medicine and John …
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