Call us toll-free

Quick academic help

Don't let the stress of school get you down! Have your essay written by a professional writer before the deadline arrives.

Calculate the price

Pages:

275 Words

$19,50

DNA Polymerase: Fidelity, Rate, Processivity - YouTube

Bacteriophage T4 gene 44/62 and 45 accessory proteins stimulate DNA synthesis by T4 DNA polymerase. Using a φX174 DNA template primed with a strand of a restriction fragment, these accessory proteins also greatly increase the hydrolysis of dNTP to dNMP which accompanies DNA synthesis by the polymerase. The rate of formation of a deoxynucleoside monophosphate is up to 10 times the rate of its incorporation into DNA. This rapid hydrolysis depends on the combined presence of the polymerase, the accessory proteins, and the template primer. Whth a φX174 template-primer, dCTP is hydrolyzed more efficiently than the other triphosphates; with activated salmon sperm DNA, dATP and dGTP are the most efficiently hydrolyzed. A likely explanation for the deoxynucleoside triphosphate hydrolysis is that the polymerase when detained at hair pin barriers in the template, will alternately incorporate and then, using its 3' exonuclease activity, remove deoxynucleoside monophosphates. The formation of deoxynucleoside monophosphates by this mechanism is very slow in the absence of accessory proteins probably because the polymerase is less processive and frequently dissociates from the DNA when detained at a hairpin barrier. Supporting this explanation is the fact that a primer with a 3' terminus near a stable hairpin barrier will support high levels of deoxynucleoside monophosphate formation but relatively low levels of incorporation. Addition of gene 32 protein virtually eliminates the hairpin barriers, increases the rate of synthesis, and markedly reduces the rate of deoxynucleoside monophosphate formation.

The rate of DNA synthesis is 1 kb/s in E

AB - Bacteriophage T4 gene 44/62 and 45 accessory proteins stimulate DNA synthesis by T4 DNA polymerase. Using a φX174 DNA template primed with a strand of a restriction fragment, these accessory proteins also greatly increase the hydrolysis of dNTP to dNMP which accompanies DNA synthesis by the polymerase. The rate of formation of a deoxynucleoside monophosphate is up to 10 times the rate of its incorporation into DNA. This rapid hydrolysis depends on the combined presence of the polymerase, the accessory proteins, and the template primer. Whth a φX174 template-primer, dCTP is hydrolyzed more efficiently than the other triphosphates; with activated salmon sperm DNA, dATP and dGTP are the most efficiently hydrolyzed. A likely explanation for the deoxynucleoside triphosphate hydrolysis is that the polymerase when detained at hair pin barriers in the template, will alternately incorporate and then, using its 3' exonuclease activity, remove deoxynucleoside monophosphates. The formation of deoxynucleoside monophosphates by this mechanism is very slow in the absence of accessory proteins probably because the polymerase is less processive and frequently dissociates from the DNA when detained at a hairpin barrier. Supporting this explanation is the fact that a primer with a 3' terminus near a stable hairpin barrier will support high levels of deoxynucleoside monophosphate formation but relatively low levels of incorporation. Addition of gene 32 protein virtually eliminates the hairpin barriers, increases the rate of synthesis, and markedly reduces the rate of deoxynucleoside monophosphate formation.

Low-fidelity DNA synthesis by human DNA polymerase …

The role of dna polymerase during dna synthesis is that the dna polymerase synthesize the dna molecules during dna synthesis in which they contribute to the ..

N2 - Bacteriophage T4 gene 44/62 and 45 accessory proteins stimulate DNA synthesis by T4 DNA polymerase. Using a φX174 DNA template primed with a strand of a restriction fragment, these accessory proteins also greatly increase the hydrolysis of dNTP to dNMP which accompanies DNA synthesis by the polymerase. The rate of formation of a deoxynucleoside monophosphate is up to 10 times the rate of its incorporation into DNA. This rapid hydrolysis depends on the combined presence of the polymerase, the accessory proteins, and the template primer. Whth a φX174 template-primer, dCTP is hydrolyzed more efficiently than the other triphosphates; with activated salmon sperm DNA, dATP and dGTP are the most efficiently hydrolyzed. A likely explanation for the deoxynucleoside triphosphate hydrolysis is that the polymerase when detained at hair pin barriers in the template, will alternately incorporate and then, using its 3' exonuclease activity, remove deoxynucleoside monophosphates. The formation of deoxynucleoside monophosphates by this mechanism is very slow in the absence of accessory proteins probably because the polymerase is less processive and frequently dissociates from the DNA when detained at a hairpin barrier. Supporting this explanation is the fact that a primer with a 3' terminus near a stable hairpin barrier will support high levels of deoxynucleoside monophosphate formation but relatively low levels of incorporation. Addition of gene 32 protein virtually eliminates the hairpin barriers, increases the rate of synthesis, and markedly reduces the rate of deoxynucleoside monophosphate formation.

DNA polymerase III holoenzyme - Wikipedia

Full-text (PDF) | High-fidelity DNA synthesis conditions are those that exploit the inherent ability of polymerases to discriminate against errors

11/16/2016 · Molecular Biology: Processivity, Rate, and Fidelity in DNA Polymerase - Duration: 7:20
Order now
  • UNMATCHED QUALITY

    As soon as we have completed your work, it will be proofread and given a thorough scan for plagiarism.

  • STRICT PRIVACY

    Our clients' personal information is kept confidential, so rest assured that no one will find out about our cooperation.

  • COMPLETE ORIGINALITY

    We write everything from scratch. You'll be sure to receive a plagiarism-free paper every time you place an order.

  • ON-TIME DELIVERY

    We will complete your paper on time, giving you total peace of mind with every assignment you entrust us with.

  • FREE CORRECTIONS

    Want something changed in your paper? Request as many revisions as you want until you're completely satisfied with the outcome.

  • 24/7 SUPPORT

    We're always here to help you solve any possible issue. Feel free to give us a call or write a message in chat.

Order now
  • You submit your order instructions

  • We assign an appropriate expert

  • The expert takes care of your task

  • We send it to you upon completion

Order now
  • 37 684

    Delivered orders

  • 763

    Professional writers

  • 311

    Writers online

  • 4.8/5

    Average quality score

Order now
  • Kim

    "I have always been impressed by the quick turnaround and your thoroughness. Easily the most professional essay writing service on the web."

  • Paul

    "Your assistance and the first class service is much appreciated. My essay reads so well and without your help I'm sure I would have been marked down again on grammar and syntax."

  • Ellen

    "Thanks again for your excellent work with my assignments. No doubts you're true experts at what you do and very approachable."

  • Joyce

    "Very professional, cheap and friendly service. Thanks for writing two important essays for me, I wouldn't have written it myself because of the tight deadline."

  • Albert

    "Thanks for your cautious eye, attention to detail and overall superb service. Thanks to you, now I am confident that I can submit my term paper on time."

  • Mary

    "Thank you for the GREAT work you have done. Just wanted to tell that I'm very happy with my essay and will get back with more assignments soon."

Ready to tackle your homework?

Place an order