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Coupling efficiency is important because the effects are cumulative during DNA synthesis. The Table below shows the effect of a 1% difference in coupling efficiency and how this influences the amount of full-length product, following the synthesis of oligos of different length. Considering a relatively short oligo of 20 bases, a 1% difference in coupling efficiency can result in a 15% difference, in terms of full-length final product.

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For the 0.2 µmol synthesis scale, monomer coupling is done at a 40-50-fold excess. Doing so for larger scale synthesis (such as for a 1.0 µmol scale) would be cost-prohibitive. Large-scale syntheses are done only at 10-fold mole excess of amidites. To increase the final yields of these large-scale synthesis, the coupling times are extended, so to increase coupling efficiencies.

custom oligo synthesis, oligonucleotides ..

Oligo DNAsynthesis

For oligos of standard length (≤ 33 nucleotides) we guarantee a certain yield of DNA (OD260 applying to a 20-mer) to be delivered according to the respective scale of synthesis and mode of purification.

Oligos are made using a DNA synthesizer, which is basically a computer-controlled reagent delivery system. The first base is attached to a solid support, usually a glass or polystyrene bead, which is designed to anchor the growing DNA chain in the reaction column. DNA synthesis consists of a series of chemical reactions.

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Oligo DNAsynthesis

The trityl-group is colorless when attached to a DNA base but it gives a characteristic orange color once removed. The intensity of this color can be measured by UV spectrophotometry and it is directly related to the number of trityl molecules present. Following the first coupling step, the amount of trityl released during deblocking is directly proportional to the amount of full-length oligo synthesized in the previous cycle. When the trityl is cleaved during the deblocking step, the resulting trityl cation is orange in color. The intensity of this color can be measure by UV spectrophotometry. By comparing the intensities of the trityl cation produced after the first and last coupling steps, one can calculate the average successful base coupling per cycle and hence the coupling efficiency.

DNA synthesis is a complicated process, which has improved significantly over the last years. Despite these improvements, all manufacturers have an inherent failure rate. We are constantly developing our processes and systems to minimize these losses; however, it is inevitable that we will occasionally have to re-synthesize some oligos. Please note that metabion performs strict quality controls on each and every oligo synthesized. If an oligo does not pass our quality tests, it will be resynthesized.

Oligo DNA synthesis
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Oligo Analysis Tool - Eurofins Genomics

S-Oligo DNA is olig DNA whose phosphate phosphate groups between nucleotides are phosphorothioated. This Oligo DNA is so tolerant against most nucleases that they are used for the experiments of anti-sense.

Oligo Analyzer – Integrated DNA Technologies

With over 20 years of experience in oligo synthesis, Metabion can provide you with a full range of DNA oligonucleotides and modifications of highest quality at competitive prices!

Premium DNA Oligonucleotide Synthesis: ..

The company has all the internationally leading core technologies of siRNA chemical synthesis, which include the RNA monomer synthesis technology, the custom and chemically-modified siRNA oligo synthesis technology, the nucleic acid fluorescent labeling technology, and many nucleotide chemical modification technologies.

Custom PNA Oligo Synthesis Service | PNA BIO

Place your order through this website or by email or by calling us to discuss the specific technical challenges your research faces and the custom oligos we will synthesize just for you.

Products & Service / Custom Oligo synthesis_Mebep Bio …

purification is standard (no additional charge) for all our dual-labeled probes and/or multi-labeled oligos, as well as for all our RNA and large scale oligos.

Custom Oligo Synthesis - General Biosystems

As the largest gene synthesis provider, we provides standard oligos and custom modified DNA oligos with comprehensive modifications and labeling to meet research needs in biology, diagnostics, and drug discovery.

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Gene Universal, located in Delaware USA, is a pioneer in the field of gene synthesis and synthetic biology. As a leading company in genomics research and applied biological technology, and with our strong technical force, unparalleled customized service and fast delivery time, we are aiming to provide more cost-effective service and product to accelerate the global research in drug discovery, biology optimization, food and agriculture, etc.

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