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Collagen: Types and Synthesis Pathway - YouTube
Chojkier, M., Spanheimer., R., and Peterkofski, B., 1983, Specifically decreased procollagen biosynthesis in scurvy dissociated from an effect on proline hydroxylation and correlated with body weight loss, J. Clin. Invest. 72:826-835.
The total RNA of cells was isolated using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) and subjected to reverse transcription with Oligo (dT) and M-MLV Reverse Transcriptase (Thermo Fisher Scientific). Synthesized complementary DNA (cDNA) was analysed with quantitative real-time PCR using SYBR® Premix (Takara, Dalian, China) and Roche480 system. Glyceraldehyde 3-phospate dehydrogenase (GAPDH) was used as a reference gene. Primers sequences were as follows: collagen, type I, alpha 2 (Col1a2; mouse), 5 -GGTGAGCCTGGTCAAACGG-3 (forward) and 5 - ACTGT-GTCCTTTCACGCCTTT-3 (reverse); Col1a2 (human), 5 -GGCCCTCAAGGTTTCCAAGG-3 (forward) and 5 -CACCCTGTGGTCCAACAACTC-3 (reverse); collagen, type III, alpha 1 (Col3a1; mouse), 5 - CTGTAACATGGAAACTGGGGAAA-3 (forward) and 5 - CCATAGCTGAACTGAAAACCACC-3 (reverse); Col3a1 (human), 5 -TTGAAGGAGGATGTTCCCATCT-3 (forward) and 5 - ACAGACACATATTTGGCATGGTT-3 (reverse); matrix metalloproteinases 1 (MMP1; human), 5 -AAAATTACACGCCAGATTTGCC-3 (forward) and 5 -GGTGTGACATTACTCCAGAGTTG-3 (reverse); matrix metalloproteinases 2 (MMP2; human), 5 -TACAGGATCATTGGCTACACACC-3 (forward) and 5 -GGTCACATCGCTCCAGACT-3 (reverse); matrix metalloproteinases 9 (MMP9; human), 5 -TGTACCGCTATGGTTACACTCG-3 (forward) and 5 -GGCAGGGACAGTTGCTTCT-3 (reverse); tissue inhibitor of metalloproteinases1 (TIMP1; human), 5 -CTTCTGCAATTCCGACCTCGT-3 (forward) and 5 -ACGCTGGTATAAGGTGGTCTG-3 (reverse); α-SMA (human), 5 -AAAAGACAGCTACGTGGGTGA-3 (forward) and 5 -GCCATGTTCTATCGGGTACTTC-3 (reverse).
Proline Precursors to Sustain Mammalian Collagen Synthesis
Senoo, H., and Hata, R., 1994, Extracellular matrix regulates and L-ascorbic acid 2-phosphate further modulates morphology, proliferation, and collagen synthesis of perisinusoidal stellate cells. Biochem. Biophys. Res. Commun. 200:999-1006.
Varani J, Warner RL, Gharaee-Kermani M,Phan SH, Kang S, Chung JH, Wang ZQ, Datta SC, Fisher GJ andVoorhees JJ: Vitamin A antagonizes decreased cell growth andelevated collagen-degrading matrix metalloproteinases andstimulates collagen accumulation in naturally aged human skin. JInvest Dermatol. 114:480–486. 2000. : :
Collagen synthesis,Types and Composition (Part -2) ..
Choi MS, Yoo MS, Son DJ, Jung HY, Lee SH,Jung JK, Lee BC, Yun YP, Pyo HB and Hong JT: Increase of collagensynthesis by obovatol through stimulation of the TGF-beta signalingand inhibition of matrix metalloproteinase in UVB-irradiated humanfibroblast. J Dermatol Sci. 46:127–137. 2007. : :
Fibroblast overactivation leads to pathological collagen deposition or scar formation. Myofibroblasts, known as activated fibroblasts, are marked by α-SMA expression. To determine the effects of apigenin on fibroblasts’ activation, we evaluated the levels of α-SMA mRNA and protein expression () in cultured HDFs treated with apigenin. We found that α-SMA mRNA expression had no obvious change in apigenin-treated cells compared with DMSO-treated cells. We also showed that apigenin did not affect α-SMA expression in vitro by immunofluorescence staining (). These findings suggested that apigenin did not cause fibroblasts to overactivate into myofibroblasts while collagen synthesis was increasing.
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Apigenin Induces Dermal Collagen Synthesis Via ..
Gould, B.S., and Woessner, J.F., 1957, Biosynthesis of collagen. The influence of ascorbic acid on the proline, hydroxyproline, glycine and collagen content of regenerating guinea pig skin, J. Biol. Chem. 266:289-300.
Pyropia yezoensis peptide promotes collagen synthesis …
Gould, B.S., 1958, Biosynthesis of collagen. III. The direct action of ascorbic acid on hydroxyproline and collagen formation in subcutaneous polyvinyl sponge implants in guinea pigs, J. Biol. Chem. 232:637-649.
Collagen IV synthesis is restricted to the …
MMP-13 plays an important role in the degradation of collagen . This study showed that, MMP-13 transcription an protein content were increased by Zn added and this significantly inhibited the expression of αSMA and type I collagen. MMP-13 is a matrix metalloproteinase involved in the regulation of hepatic fibrosis. This study failed to detect the influence of Zn on the expression of other MMPs, however, related experiments will be carried out in follow-up works. TIMP1 plays an inhibitory effect on matrix metalloproteinases, imbalance between them is an important reason that leads to liver fibrosis, in this study, it was shown that Zn did not affect the protein content of TIMP1. These results demonstrated that MMP-13 played a key role in the process of collagen synthesis inhibition by Zn in LX-2 cells.
To measure collagen synthesis, ..
Cosiewska, A., Wilson, S., Kwon, D., and Peterkowsky, B., 1994, Evidence for an in vivo role of insulin-like growth factor-binding protein-1 and -2 as inhibitors of collagen gene expression in vitamin C-deficient and fasted guinea pigs, Endocrinology 134:1329-1339.
Apigenin induces dermal collagen synthesis via …
Yoon JH, Kim J, Lee H, Kim SY, Jang HH,Ryu SH, Kim BJ and Lee TG: Laminin peptide YIGSR induces collagensynthesis in Hs27 human dermal fibroblasts. Biochem Biophys ResCommun. 428:416–421. 2012. : :
Collagen - Biochemistry - Medbullets Step 1
Geesin, J.C., Brown, L.J., Gordon, J.S., and Berg, R.A., 1993, Regulation of collagen synthesis in human dermal fibroblasts in contracted collagen gels by ascorbic acid, growth factors and inhibitors of lipid peroxidation, Exp. Cell Res. 206:283-290.
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