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Mint-2 cDNA synthesis kit - EVN-SK005 - Axxora
Available for both SYBR® Green and TaqMan detection, One-Step PrimeScript RT-PCR Kits allow all RT-PCR steps to be performed in a single tube, simplifying reaction set-up, minimizing the risk of contamination, and, with a reverse transcription step of just 5 minutes, allowing rapid analysis. In Figure 2, the was used to analyze HPRT1 expression in human liver total RNA (2 pg–2 µg) in a single-tube reaction (cDNA synthesis and real-time PCR performed in tandem). This one-step protocol using PrimeScript RTase allowed high sensitivity and specificity detection of HPRT1 mRNA across a wide range of template RNA concentrations (Figure 2).
Trimmer-2 kit is designed to normalize amplified full-length-enriched cDNA prepared using . The resulting cDNA contains equalized abundance of different transcripts and can be used for construction of cDNA libraries and for direct sequencing, including high-throughput sequencing on the next generation sequencing platforms (Roche/454, ABI/SOLiD or Illumina/Solexa). The kit also includes special adapters allowing use of Clontech SMART-based kits for construction of cDNA intended for Trimmer-2 normalization.
OneScript ® cDNA Synthesis Kit ..
Ordinarily, it can take 30 to 60 minutes to synthesize cDNA templates for real-time RT-PCR (reverse-transcription PCR). With PrimeScript Reverse Transcriptase, RT reactions can be completed in as few as 15 minutes. Not fast enough? Use a one-step PrimeScript kit and set your reverse transcription step to just 5 minutes.
When compared with a first-strand cDNA synthesis kit from Company L, the PrimeScript RT Reagent Kit synthesized cDNA templates from human total RNA with a higher RT efficiency and provided a significant time-savings benefit (Figure 1 and Table I). The PrimeScript RT Reagent kit provided higher yields of cDNA, resulting in lower Ct values (Table I). In addition, the PrimeScript kit provided a significant time savings, synthesizing real-time PCR-ready cDNA templates in just 15 minutes as opposed to over an hour as was required for the Company L kit.
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Total RNA was extracted from approximately 30 mg oftumor tissues obtained by TURBT using TRIzol RNA Isolation Reagent(Life Technologies Inc., Rockville, MD, USA) and wasreverse-transcribed to cDNA using a SuperScript VILO cDNA SynthesisKit (Life Technologies Inc.).
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