BHA had no effect on the growth of the animals.

Liver cytosols prepared from BHA-fed female CD-1 mice or male Sprague-Dawley rats reduced the mutagenicity of urinary benzo(a) pyrene metabolites as tested in S.

However, microsomal preparations from the BHA-fed mice showed greater sensitivity to in vitro inhibition of AHH activity by alpha-naphtha- fluorine, and continued increased amounts of cytochrome P₄₅₀ per unit weight than preparations from control mice (Speier & Wattenberg, 1975).

BHA had no effect on these enzyme activities.

Rats administered BHA (500 mg/kg day) for two days showed no increased activity of microsomal processing enzymes (aminopyrine demethylase) hexabarbitine oxidase and nitroanisol demethylase (Gilbert & Goldberg, 1965).

In another study, rats fed 0.1, 0.25 or 0.5% BHA in the diet for 12 days showed no increased liver weight, but there was an increase in liver biphenyl-4-hydroxylase activity in the 0.5% group (Creaven et al., 1966).

BHA alone did not cause these changes (Riley & Seal, 1968).

BHA at levels of 400 µg/ml caused an inhibition of the metabolism (as measured by gas evolution) of culture of bacteria isolated from the faecal flora of rats (Fritsch et al., 1975b).

Combinations of BHA with other food additives, such as chlorine dioxide, sodium propionate, propyl gallate, or polyoxyethylene-8- stearate, at 50 times the normal levels of use in bread, had no deleterious effects when they were fed in bread to groups of 26 rats for a period of 32 weeks.

BHA was non-mutagenic for Salmonella TA-1530 and G-46.

Two of the possible responding patients who were being treated for eczema remained symptom-free on a diet free of antioxidants but redeveloped eczema upon challenge with 5 or 10 mg of BHA daily given orally.

172.110 BHA.Subpart B--Food Preservatives

Seven patients suffering from asthma or rhinitis of unknown etiology were challenged with 300-450 mg of BHA given orally, except one patient who received 850 mg.

BHA produced random statistical increases in dominant lethality.

For example, it was reported that BHA suppressed the in vitro immune response of spleen cells at concentrations that were not cytotoxic (Archer et al., 1977).

182.3173 Butylated hydroxytoluene.

Special studies on carcinogenicity BHA was tested for its ability to induce lung tumours when given to groups of 15 male and 15 female strain A mice in series of 24 i.p.

Inter-subject variability in blood levels of BHA was large.

The BHA-treated offspring showed increased exploration, decreased sleeping, decreased self-grooming, slower learning and a decreased orientation complex than did the control group (Stokes & Scudder, 1974).

BHA had no effect on any of the indices tested.

In a study carried out using groups of 30 CD-1 female mice, aged 6-8 weeks, BHA did not promote skin tumours when applied to the skin of animals previously initiated with 7,12-dimethyl-benzanthracene (DMBA).

* As BHA, BHT, TBHQ or the sum of the three compounds.

Special studies on reproduction and behaviour Diets containing 0, 0.125, 0.25 or 0.5% BHA were fed to adult male and female Sprague-Dawley rats for two weeks prior to and during mating.

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