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Using one of those sixstrategies, generate5 a research hypothesis.

Genomics has profoundly changed biology by scaling data acquisition, which has provided researchers with the opportunity to interrogate biology in novel and creative ways. No longer constrained by low-throughput assays, researchers have developed hypothesis-generating approaches to understand the molecular basis of nature—both normal and pathological. The paradigm of hypothesis-generating research does not replace or undermine hypothesis-testing modes of research; instead, it complements them and has facilitated discoveries that may not have been possible with hypothesis-testing research. The hypothesis-generating mode of research has been primarily practiced in basic science but has recently been extended to clinical-translational work as well. Just as in basic science, this approach to research can facilitate insights into human health and disease mechanisms and provide the crucially needed data set of the full spectrum of genotype–phenotype correlations. Finally, the paradigm of hypothesis-generating research is conceptually similar to the underpinning of predictive genomic medicine, which has the potential to shift medicine from a primarily population- or cohort-based activity to one that instead uses individual susceptibility, prognostic, and pharmacogenetic profiles to maximize the efficacy and minimize the iatrogenic effects of medical interventions.

Hypothesis in Qualitative Research - ResearchGate

As in the basic and translational research discussion above, the question could be raised as to whether the differential diagnostic paradigm is necessary for genetic disorders. Indeed, the availability of clinical genome and exome sequencing heralds an era when the test could be ordered relatively early in the diagnostic process, with the clinician serving in a more interpretative role, rather than as a test selector (). This approach has already been adopted for copy number variation, because whole genome array CGH- or SNP-based approaches have mostly displaced more specific single-gene or single-locus assays and standard chromosome analyses (). But the paradigm can be taken beyond hypothesis-generating clinical diagnosis into predictive medicine. One can now begin to envision how whole genome approaches could be used to assess risks prospectively for susceptibility to late-onset disorders or occult or subclinical disorders. The heritable cancer susceptibility syndromes are a good example of this. The current clinical approach is to order a specific gene test if a patient presents with a personal history of an atypical or early-onset form of a specific cancer syndrome, or has a compelling family history of the disease. As in the prior examples, this is because individual cancer gene testing is expensive and low throughput. One can ask the question whether this is the ideal approach or if we could be screening for these disorders from genome or exome data. Again, we applied sequencing analysis for these genes to the ClinSeq cohort because they were not ascertained for that phenotype. In a published study of 572 exomes (), updated here to include 850 exomes, we have identified 10 patients with seven distinct cancer susceptibility syndrome mutations. These were mostly familial breast and ovarian cancer (BRCA1 and BRCA2), with one patient each with paraganglioma and pheochromocytoma (SDHC) and one with Lynch syndrome (MSH6). What is remarkable about these diagnoses is that only about half of them had a convincing personal or family history of the disease, and thus most would have not been offered testing using the current, hypothesis-testing clinical paradigm. These data suggest that screening for these disorders using genome or exome sequencing could markedly improve our ability to identify such families before they develop or die from these diseases—the ideal of predictive genomic medicine.

Deduction & Induction - Social Research Methods

An experiment is a procedure carried out to support, refute, or validate a hypothesis

The classical paradigm for basic biological research has been to develop a specific hypothesis that can be tested by the application of a prospectively defined experiment (see ). I suggest that one of the major (although not the only) factors that led to the development of this paradigm is that experimental design was limited by the throughput of available assays. This low throughput mandated that the scientific question had to be focused narrowly to make the question tractable. However, the paradigm can be questioned if the scientist has the ability to assay every potential attribute of a given type (e.g., all genes). If the hypothesis is only needed to select the assay, one does not need a hypothesis to apply a technology that assays all attributes. In the case of sequencing, the radical increase in throughput can release scientists from the constraint of the specific hypothesis because it has allowed them to interrogate essentially all genotypes in a genome in a single assay. This capability facilitates fundamental biological discoveries that were impossible or impractical with a hypothesis-testing mode of scientific inquiry. Examples of this approach are well demonstrated by several discoveries that followed the sequencing of a number of genomes. An example was the discovery that the human gene count was just over 20,000 (), much lower than prior estimates. This result, although it was much debated and anticipated, was not a hypothesis that drove the human genome project, but nonetheless was surprising and led to insights into the nuances of gene regulation and transcriptional isoforms to explain the complexity of the human organism. The availability of whole genome sequence data from multiple species facilitated analyses of conservation. While it was expected that protein-coding regions, and to a lesser extent promoters and 5′- and 3′-untranslated regions of genes, would exhibit recognizable sequence conservation, it was unexpected that an even larger fraction of the genomes outside of genes are highly conserved (). This surprising and unanticipated discovery has spawned a novel field of scientific inquiry to determine the functional roles of these elements, which are undoubtedly important in physiology and pathophysiology. These discoveries demonstrate the power of hypothesis-generating basic research to illuminate important biological principles.

The approach to clinical research grew out of the basic science paradigm as described above. The first few steps of selecting a scientific problem and developing a hypothesis are similar, with the additional step () of rigorously defining a phenotype and then carefully selecting research participants with and without that trait. As in the basic science paradigm, the hypothesis is tested by the application of a specific assay to the cases and controls. Again, this paradigm has been incredibly fruitful and should not be abandoned, but the hypothesis-generating approach can be used here as well. In this approach, a cohort of participants is consented, basic information is gathered on their health, and then a high-throughput assay, such as genome or exome sequencing, is applied to all of the participants. Again, because the assay tests all such attributes, the research design does not necessitate a priori selections of phenotypes and genes to be interrogated. Then, the researcher can examine the sequence data set for patterns and perturbations, form hypotheses about how such perturbations might affect the phenotype of the participants, and test that hypothesis with a clinical research evaluation. This approach has been used with data from genome-wide copy number assessments (array CGH and SNP arrays), but sequencing takes it to a higher level of interrogation and provides innumerable variants with which to work.

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Despite these optimistic scenarios and examples, it remains true that our ability to perform true predictive medicine is limited. These limitations include technical factors such as incomplete sequence coverage, imperfect sequence quality, inadequate knowledge regarding the penetrance and expressivity of most variants, uncertain medical approaches and utility of pursuing variants from genomic sequencing, and the poor preparation of most clinicians for addressing genomic concerns in the clinic (). Recognizing all of these limitations, it is clear that we are not prepared to launch broad-scale implementation of predictive genomic medicine, nor should all research be structured using the hypothesis-generating approach.

An example of this type of sequence-based hypothesis-generating clinical research started with a collaborative project in which we showed that mutations in the gene ACSF3 caused the biochemical phenotype of combined malonic and methylmalonic acidemia (). At that time, the disorder was believed to be a classic pediatric, autosomal-recessive severe metabolic disorder with decompensation and sometimes death. We then queried the ClinSeq cohort () to assess the carrier frequency, to estimate the population frequency of this rare disorder. Because ClinSeq is a cohort of adults with a range of atherosclerosis severity, we reasoned that this would serve as a control population for an unbiased estimate of ACSF3 heterozygote mutant alleles. Surprisingly, we identified a ClinSeq participant who was homozygous for one of the mutations identified in the children with the typical phenotype. Indeed, one potential interpretation of the data would be that the variant is, in fact, benign and was erroneously concluded to be pathogenic, based on finding it in a child with the typical phenotype. It has been shown that this error is common, with up to 20% of variants listed in databases as pathogenic actually being benign (). Further clinical research on this participant led to the surprising result that she had severely abnormal blood and urine levels of malonic and methylmalonic acid (). This novel approach to translational research was a powerful confirmation that the mutation was indeed pathogenic, but there was another, even more important conclusion. We had conceptualized the disease completely incorrectly. Instead of being only a severe, pediatric metabolic disorder, it was instead a disorder with a wide phenotypic spectrum in which one component of the disease is a metabolic perturbation and another component is a susceptibility to severe decompensation and strokes. This research indeed raises many questions about the natural history of the disorder, whether the pediatric decompensation phenotype is attributable to modifiers, what the appropriate management of such an adult would be, etc.

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What Is a Scientific Hypothesis? | Definition of Hypothesis

Hypothesis-testing approaches to science and medicine have served us well and should continue. However, the advent of massively parallel sequencing and other high-throughput technologies provides opportunities to undertake hypothesis-generating approaches to science and medicine, which in turn provide unprecedented opportunities for discovery in the research realm. This can allow the discovery of results that were not anticipated or intended by the research design, yet provide critical insights into biology and pathophysiology. Similarly, hypothesis-generating clinical research has the potential to provide these same insights and, in addition, has the potential to provide us with data that will illuminate the full spectrum of genotype–phenotype correlations, eliminating the biases that have limited this understanding in the past. Finally, applying these principles to clinical medicine can provide new pathways to diagnosis and provide the theoretical basis for predictive medicine that can detect disease susceptibility and allow health to be maintained, instead of solely focusing on the treatment of evident disease.

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