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What is needed for photosynthesis to occur? | …

To determinewhich of these original compounds contributed to the O2 endproduct, an isotopic tracer experiment was performed using 18O:

Now we need to understand how cells can use the products ofphotosynthesis to obtain energy.
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In the presence of the enzyme Rubisco,one molecule of CO2 is combined with one molecule of RuBP, and the first product of this reaction is two molecules of PGA.

What is needed for photosynthesis to occur

In addition to theenergy released, the products of the reaction are carbon dioxide and water.
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If the light intensity is not a limiting factor, there will usually be a shortage of NADP+ as NADPH accumulates within the stroma (see light independent reaction). NADP+ is needed for the normal flow of electrons in the thylakoid membranes as it is the final electron acceptor. If NADP+ is not available then the normal flow of electrons is inhibited. However, there is an alternative pathway for ATP production in this case and it is called cyclic photophosphorylation. It begins with Photosystem I absorbing light and becoming photoactivated. The excited electrons from Photosystem I are then passed on to a chain of electron carriers between Photosystem I and II. These electrons travel along the chain of carriers back to Photosystem I and as they do so they cause the pumping of protons across the thylakoid membrane and therefore create a proton gradient. As explained previously, the protons move back across the thylakoid membrane through ATP synthase and as they do so, ATP is produced. Therefore, ATP can be produced even when there is a shortage of NADP+.

Immediatelyafter exposure to 14CO2, the plant's photosynthetic tissue iskilled by immersing it in boiling alcohol, and all of the biochemical reactions cease.

Plants get the water needed for photosynthesis from their roots

14CO2 is exposed for a brief period to a green plantthat is conducting a photosynthesis in the presence of sunlight.
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During carbon fixation, carbon dioxide in the stroma (which enters the chloroplast by diffusion) reacts with a five-carbon sugar called ribulose bisphosphate (RuBP) to form a six-carbon compound. This reaction is catalysed by an enzyme called ribulose bisphosphate carboxylase (large amounts present within the stroma), otherwise known as rubisco. As soon as the six-carbon compound is formed, it splits to form two molecules of glycerate 3-phosphate. Glycerate 3-phosphate is then used in the reduction reactions.

The light-independant reactions of photosynthesis occur in the stroma of the chloroplast and involve the conversion of carbon dioxide and other compounds into glucose. The light-independent reactions can be split into three stages, these are carbon fixation, the reduction reactions and finally the regeneration of ribulose bisphosphate. Collectively these stages are known as the Calvin Cycle.

This continued use of pyruvic acid during fermentation permitsglycolysis to continue with its associated production of ATP.
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A Compound Needed for Photosynthesis;

A limiting factor is a factor that controls a process. Light intensity, temperature and carbon dioxide concentration are all factors which can control the rate of photosynthesis. Usually, only one of these factors will be the limiting factor in a plant at a certain time. This is the factor which is the furthest from its optimum level at a particular point in time. If we change the limiting factor the rate of photosynthesis will change but changes to the other factors will have no effect on the rate. If the levels of the limiting factor increase so that this factor is no longer the furthest from its optimum level, the limiting factor will change to the factor which is at that point in time, the furthest from its optimum level. For example, at night the limiting factor is likely to be the light intensity as this will be the furthest from its optimum level. During the day, the limiting factor is likely to switch to the temperature or the carbon dioxide concentration as the light intensity increases.

A gaseous compound of carbon and ..

As we can see, there is a close relationship between the action spectrum and absorption spectrum of photosynthesis. There are many different types of photosynthetic pigments which will absorb light best at different wavelengths. However the most abundant photosynthetic pigment in plants is chlorophyll and therefore the rate of photosynthesis will be the greatest at wavelengths of light best absorbed by chlorophyll (400nm-525nm corresponding to violet-blue light). Very little light is absorbed by chlorophyll at wavelengths of light between 525nm and 625 (green-yellow light) so the rate of photosynthesis will be the least within this range. However, there are other pigments that are able to absorb green-yellow light such as carotene. Even though these are present in small amounts they allow a low rate of photosynthesis to occur at wavelengths of light that chlorophyll cannot absorb.

and which is needed by plants for photosynthesis

An absorption spectrum is a graph showing the percentage of light absorbed by pigments within the chloroplast, for each wavelength of light. An example is the absorption spectrum of chlorophyll a and b. The best absorption is seen with violet-blue light. There is also good absorption with red-orange light. However most of the green-yellow light is reflected and therefore not absorbed. This wavelength of light shows the least absorption.

The raw materials of photosynthesis, ..

In respiration energy is released fromsugars when electrons associated with hydrogen are transported to oxygen (theelectron acceptor), and water is formed as a byproduct. The mitochondriause the energy released in this oxidation in order to synthesize ATP. Inphotosynthesis, the electron flow is reversed, the water is split (not formed),and the electrons are transferred from the water to CO2 and in theprocess the energy is used to reduce the CO2 into sugar. Inrespiration the energy yield is 686 kcal per mole of glucose oxidized to CO2,while photosynthesis requires 686 kcal of energy to boost the electrons from thewater to their high-energy perches in the reduced sugar -- light provides thisenergy.

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